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Phusion Dna Polymerase Tm Calculator. Anneal at 3c above the lowest tm if the primers are longer than 20nt, else at the tm. • 50 mm mgcl 2 solution.
The Elk Crew—Biocatalysis at the University of Graz from mecp12.uni-graz.at
Phusion dna polymerase (2u/μ) 0.5μl. Use the neb tm calculator to estimate an appropriate annealing temperature when using neb pcr products. • 5x phusion green hf buffer.
For Optimal Results Start By Accurately Calculating Your Tm With Our Tm Calculator.
It eliminates the need for a separate dna purification. Not for use in diagnostic procedures. Anneal at 3c above the lowest tm if the primers are longer than 20nt, else at the tm.
(≤ 20 Nt) When Calculated With Our Tm Calculator.
The unique structure and characteristics of phusion dna polymerase. Use the neb tm calculator to estimate an appropriate annealing temperature when using neb pcr products. Please note that protocols with phusion dna polymerase may differ from.
Both Phusion Hf Buffer And Phusion Gc.
For optimal results start by accurately calculating your tm with our tm calculator. Select a value from q5, q5 hot start, q5u hot. • 50 mm mgcl 2 solution.
Both phusion green hf and phusion. The neb tm calculator should be used to. Use the neb tm calculator to estimate an appropriate annealing temperature when using neb pcr products.
Optimal Annealing Temperatures For Phusion Hot Start Flex Dna Polymerase Tend To Be Higher Than For Other Pcr Polymerases.
• 50 mm mgcl 2. • 5x phusion gc buffer. Phusion dna polymerase (2u/μ) 0.5μl.
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